iSPOT
Our research is focused on the biophysics and drug discovery of Estrogen Receptor (ER), a molecular driver of breast cancer. Technologically, we have established a multi-technique data integration platform, termed iSPOT, for structure determination of protein-protein interaction and structural disorder. Currently, we center our experimental-computational studies on (1) molecular cross-talk between ER domains, (2) targeted disruption of ER domain cross-talk for drug discovery, (3) structural disorder of the ER transactivation domain, and equally if not more importantly, (4) techonolgical developement of the multi-technique data integration platform for structural biology of protein-protein interaction (PPI) and intrinsically disordered protein (IDP). Our research benefits from excellent resources to which we have access, and close collaboration with laboratories here at Cleveland and other parts of the globe.
Nov. 2, 2018: Our discovery on estrogen receptor is highlighted at the Advanced Photon Source of Argonne National Laboratory; see this article: "This Boot was Made for Crosstalk".
October, 2018: A concept of "Contact Metastability" is established and validated for protein disorder in the ER transactivation domain, which is accepted for publication in the journal of Structure. This "order out of disorder" is found to be critical for the receptor's structure and function. Specifically, disruption of a metastable contact by mutagenesis alters ensemble-structures and coactivator binding. see the preprint.
Oct. 10, 2018: Our discovery on estrogen receptor is reported in the press release: "New options for breast cancer drug development found in estrogen receptors".
Aug. 8, 2018: The first structure of a multidomain estrogen receptor, enabled by our iSPOT technology, is published in Nature Communications! Read more ...
May 19, 2017: We are launching a prototype of iSPOT . Stay tuned and thanks Marc!
Jan. 11, 2017: S.Y. is invited to speak at the 2017 symposium on The Future of Integrative Structural Biology in South Carolina.
Aug. 1, 2016: iSPOT is now published at Journal of Structural Biology!
October 1, 2015: A weblet for calculating Single-Residue Protection Factor is available online now!
May 18, 2015: S.Y. is invited to speak at the 2015 symposium on Computational Biophysics and Systems Biology.
Congratulations! Krishna is now Chief Scientific Officer of a new start-up at UC Davis.
Software: Fast-SAXS-pro, Single-Residue Protection Factor, and iSPOT
Teaching: Pharm528/BioC430