Our research is focused on the biophysics and drug discovery of estrogen receptor (ER), a dominant driver in breast cancer biology. Technologically, we have established an integrative multi-technique iSPOT platform for structure determination of protein-protein interaction and more recently, structural disorder. Currently, we center our experimental-computational studies on (1) molecular cross-talk between ER domains, (2) disruption of ER cross-talk for drug discovery, (3) structural disorder of ER transactivation domain and equally important, (4) techonolgical developement of a versatile multi-technique iSPOT platform for the structural biology of protein-protein interaction (PPI) and intrinsically disordered protein (IDP). Our research benefits from excellent resources to which we have access, and close collaboration with laboratories here at Cleveland and other parts of the globe.
October, 2018: "Order out of disorder" for the transactivtion domain of estrogen receptor is first reported and accepted for publication in the journal of Structure. We revealed that a metastable Ile33-Ser118 contact is observed to inhibit coactivator binding. Disruption by mutagenesis alters ensemble-structures and coactivator binding, providing a functional link of oncogenic Ser118 phosphorylation in breast cancer endocrine resistance. see the preprint.
Aug. 8, 2018: The first structure of a multidomain estrogen receptor, enabled by our iSPOT technology, is published in Nature Communications! See news release
May 19, 2017: We are launching a prototype of iSPOT . Stay tuned and thanks Marc!
Jan. 11, 2017: S.Y. is invited to speak at the 2017 symposium on The Future of Integrative Structural Biology in South Carolina.
Aug. 17, 2016: Good news on additional funds from the NIH! Many thanks!
Aug. 1, 2016: iSPOT is now published at Journal of Structural Biology!
October 1, 2015: A weblet for calculating Single-Residue Protection Factor is available online now!
August 1, 2015: We are awarded a 5-year NIH grant of $1.56M support, in addition to our current/previous support from DoE, DoD, and ACS. Big thanks to our team members!
May 18, 2015: S.Y. is invited to speak at the 2015 symposium on Computational Biophysics and Systems Biology.
Congratulations! Krishna is now Chief Scientific Officer of a new start-up at UC Davis.
Software: Fast-SAXS-pro, Single-Residue Protection Factor, and iSPOT
Teaching: Pharm528/BioC430